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ep54  (GenScript corporation)

 
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    Structured Review

    GenScript corporation ep54
    Ep54, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep54/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    ep54 - by Bioz Stars, 2026-04
    90/100 stars

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    Photomicrographs ‘a’ (at low power) & ‘b’ (at high power with a mean MVD of 62.5 per mm 2 per mm 2 ) show a case of WDSCC, with an IRS of 5 i.e., Positive for <t>CD146</t> expression; whereas ‘c’ (at low power) & ‘d’ (at high power with a mean MVD of 133.312 per mm 2 ) show a case of endocervical adenocarcinoma, with an IRS of 6 i.e., Positive for CD146 expression Photomicrographs ‘e’ (at low power) & ‘f’ (at high power with mean MVD of 95.81 per mm2) showing a case of LGEC with an IRS of 3 i.e., negative for MCAM expression whereas ‘g’ (at low power) & ‘h’ (at high power with a mean MVD of 147.87 per mm2) show a case of HGEC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘i’ (at low power) & ‘j’ (at high power with a mean MVD of 45.81 per mm2) showing a case of APST with an IRS of 4 i.e., negative for CD146 expression; whereas ‘k’ (at low power) & ‘l’ (at high power with a mean MVD of 197.87 per mm2) showing a case of MOC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘m’ (at low power) & ‘n’ (at high power with a mean MVD of 231.25 per mm2) show a case of squamous carcinoma of the vulva with an IRS of 6 i.e., is positive for CD146 expression. Photomicrograph ‘o’ (at low power) & ‘p’(at high power with a mean MVD of 133.312 per mm2) showing a case of STIC with an IRS of 3 i.e. Negative for CD146 expression MOC: Mucinous ovarian carcinoma, MVD: Microvascular density, IRS: Immunoreactivity score, HGEC: High grade endometrial carcinoma, APST: Atypical proliferative serous tumour, STIC: Serous tubal intraepithelial carcinoma
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    Image Search Results


    Photomicrographs ‘a’ (at low power) & ‘b’ (at high power with a mean MVD of 62.5 per mm 2 per mm 2 ) show a case of WDSCC, with an IRS of 5 i.e., Positive for CD146 expression; whereas ‘c’ (at low power) & ‘d’ (at high power with a mean MVD of 133.312 per mm 2 ) show a case of endocervical adenocarcinoma, with an IRS of 6 i.e., Positive for CD146 expression Photomicrographs ‘e’ (at low power) & ‘f’ (at high power with mean MVD of 95.81 per mm2) showing a case of LGEC with an IRS of 3 i.e., negative for MCAM expression whereas ‘g’ (at low power) & ‘h’ (at high power with a mean MVD of 147.87 per mm2) show a case of HGEC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘i’ (at low power) & ‘j’ (at high power with a mean MVD of 45.81 per mm2) showing a case of APST with an IRS of 4 i.e., negative for CD146 expression; whereas ‘k’ (at low power) & ‘l’ (at high power with a mean MVD of 197.87 per mm2) showing a case of MOC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘m’ (at low power) & ‘n’ (at high power with a mean MVD of 231.25 per mm2) show a case of squamous carcinoma of the vulva with an IRS of 6 i.e., is positive for CD146 expression. Photomicrograph ‘o’ (at low power) & ‘p’(at high power with a mean MVD of 133.312 per mm2) showing a case of STIC with an IRS of 3 i.e. Negative for CD146 expression MOC: Mucinous ovarian carcinoma, MVD: Microvascular density, IRS: Immunoreactivity score, HGEC: High grade endometrial carcinoma, APST: Atypical proliferative serous tumour, STIC: Serous tubal intraepithelial carcinoma

    Journal: Turkish Journal of Obstetrics and Gynecology

    Article Title: Evaluation of the diagnostic utility of MCAM-1 (CD146) in a group of common gynecological cancers: A case-control study

    doi: 10.4274/tjod.galenos.2024.38265

    Figure Lengend Snippet: Photomicrographs ‘a’ (at low power) & ‘b’ (at high power with a mean MVD of 62.5 per mm 2 per mm 2 ) show a case of WDSCC, with an IRS of 5 i.e., Positive for CD146 expression; whereas ‘c’ (at low power) & ‘d’ (at high power with a mean MVD of 133.312 per mm 2 ) show a case of endocervical adenocarcinoma, with an IRS of 6 i.e., Positive for CD146 expression Photomicrographs ‘e’ (at low power) & ‘f’ (at high power with mean MVD of 95.81 per mm2) showing a case of LGEC with an IRS of 3 i.e., negative for MCAM expression whereas ‘g’ (at low power) & ‘h’ (at high power with a mean MVD of 147.87 per mm2) show a case of HGEC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘i’ (at low power) & ‘j’ (at high power with a mean MVD of 45.81 per mm2) showing a case of APST with an IRS of 4 i.e., negative for CD146 expression; whereas ‘k’ (at low power) & ‘l’ (at high power with a mean MVD of 197.87 per mm2) showing a case of MOC with an IRS of 6 i.e., positive for CD146 expression. Photomicrographs ‘m’ (at low power) & ‘n’ (at high power with a mean MVD of 231.25 per mm2) show a case of squamous carcinoma of the vulva with an IRS of 6 i.e., is positive for CD146 expression. Photomicrograph ‘o’ (at low power) & ‘p’(at high power with a mean MVD of 133.312 per mm2) showing a case of STIC with an IRS of 3 i.e. Negative for CD146 expression MOC: Mucinous ovarian carcinoma, MVD: Microvascular density, IRS: Immunoreactivity score, HGEC: High grade endometrial carcinoma, APST: Atypical proliferative serous tumour, STIC: Serous tubal intraepithelial carcinoma

    Article Snippet: Primary antibody CD146 [Biogenex, Anti Human CD146 (clone EP54)] was applied, followed by washing and incubation with Polyexcel target binder and Polyexcel PolyHRP (horse radish Peroxidase).

    Techniques: Expressing

    Journal: Turkish Journal of Obstetrics and Gynecology

    Article Title: Evaluation of the diagnostic utility of MCAM-1 (CD146) in a group of common gynecological cancers: A case-control study

    doi: 10.4274/tjod.galenos.2024.38265

    Figure Lengend Snippet: Distribution of CD146 expression, MVD, MVC in gynecological cancers and benign controls of the indexed study

    Article Snippet: Primary antibody CD146 [Biogenex, Anti Human CD146 (clone EP54)] was applied, followed by washing and incubation with Polyexcel target binder and Polyexcel PolyHRP (horse radish Peroxidase).

    Techniques: Expressing

    Demographic patient characteristics (n=60).

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Demographic patient characteristics (n=60).

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques:

    Representative IHC images of CD146, survivin and Ki-67 expression. Expression of CD146 in epithelioid MPeM: (A) Negative; (B) 1+; (C) 2+; (D) 3+. Expression of CD146 in non-epithelioid MPeM: (E) Negative; (F) 1+; (G) 2+; (H) 3+. Expression of survivin in epithelioid MPeM: (I) Negative; (J) 1+; (K) 2+; (L) 3+. Expression of survivin in non-epithelioid MPeM: (M) Negative; (N) 1+; (O) 2+; (P) 3+. Expression of Ki-67 in epithelioid MPeM: (Q) Lower expression; (R) higher expression. Expression of Ki-67 in non-epithelioid MPeM: (S) Lower expression; (T) higher expression. Staining of CD146 was observed in the cytoplasm and cell membrane, staining of survivin was found in the nucleus and cytoplasm, while Ki-67 staining was nuclear. Magnification, ×400. IHC, immunohistochemistry; MPeM, malignant peritoneal mesothelioma.

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Representative IHC images of CD146, survivin and Ki-67 expression. Expression of CD146 in epithelioid MPeM: (A) Negative; (B) 1+; (C) 2+; (D) 3+. Expression of CD146 in non-epithelioid MPeM: (E) Negative; (F) 1+; (G) 2+; (H) 3+. Expression of survivin in epithelioid MPeM: (I) Negative; (J) 1+; (K) 2+; (L) 3+. Expression of survivin in non-epithelioid MPeM: (M) Negative; (N) 1+; (O) 2+; (P) 3+. Expression of Ki-67 in epithelioid MPeM: (Q) Lower expression; (R) higher expression. Expression of Ki-67 in non-epithelioid MPeM: (S) Lower expression; (T) higher expression. Staining of CD146 was observed in the cytoplasm and cell membrane, staining of survivin was found in the nucleus and cytoplasm, while Ki-67 staining was nuclear. Magnification, ×400. IHC, immunohistochemistry; MPeM, malignant peritoneal mesothelioma.

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques: Expressing, Staining, Membrane, Immunohistochemistry

    Association and differences of survivin and CD146 expression with clinicopathologic parameters and Ki-67LI in patients with malignant peritoneal mesothelioma.

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Association and differences of survivin and CD146 expression with clinicopathologic parameters and Ki-67LI in patients with malignant peritoneal mesothelioma.

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques: Expressing

    Survivin (A) CD146 (C) and Ki-67 (E) expression is absent in normal mesothelium. Survivin (B) CD146 (D) and Ki-67 (F) expression is absent in peritonitis specimens (B, D, F). Magnification ×400.

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Survivin (A) CD146 (C) and Ki-67 (E) expression is absent in normal mesothelium. Survivin (B) CD146 (D) and Ki-67 (F) expression is absent in peritonitis specimens (B, D, F). Magnification ×400.

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques: Expressing

    Univariate and multivariate analyses of factors affecting OS in patients with malignant peritoneal mesothelioma.

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Univariate and multivariate analyses of factors affecting OS in patients with malignant peritoneal mesothelioma.

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques:

    Kaplan-Meier survival curves for different predictors. (A) Ki-67 grade 2 expression; (B) CD146 grade 4 expression; (C) survivin grade 4 expression; (D) forest plot of disease-free survival hazard ratios of major subgroups (exploratory analysis). Dashed lines represent median survival. PCI, peritoneal cancer index. Treatment protocols 0, best supportive care; Treatment protocols 1, intraperitoneal chemotherapy; Treatment protocols 2, systemic chemotherapy. *P<0.05. ***P<0.001.

    Journal: Oncology Letters

    Article Title: Survivin expression as an independent predictor of overall survival in malignant peritoneal mesothelioma

    doi: 10.3892/ol.2020.11505

    Figure Lengend Snippet: Kaplan-Meier survival curves for different predictors. (A) Ki-67 grade 2 expression; (B) CD146 grade 4 expression; (C) survivin grade 4 expression; (D) forest plot of disease-free survival hazard ratios of major subgroups (exploratory analysis). Dashed lines represent median survival. PCI, peritoneal cancer index. Treatment protocols 0, best supportive care; Treatment protocols 1, intraperitoneal chemotherapy; Treatment protocols 2, systemic chemotherapy. *P<0.05. ***P<0.001.

    Article Snippet: Specimens were incubated overnight at 4°C with primary antibodies against the following molecules: Survivin (dilution, 1:100; rabbit monoclonal, clone EP119; OriGene Technologies, Inc.; cat. no. S1130), CD146 (dilution, 1:100; rabbit monoclonal, clone EP54; OriGene Technologies, Inc.; cat. no. GTX34461) and Ki-67 (dilution, 1:200; rabbit monoclonal, clone EP5; OriGene Technologies, Inc.; cat. no. GTX16667).

    Techniques: Expressing